EVALUATION OF THE PROPERTIES OF THE FUCOIDAN/Fe3O4 NANOCOMPOSITE AS A TRANSPORT AGENT OF COVALENTLY BOUND MOLECULAR CARGO

Abstract

Magnetically controlled transport of drugs with targeted release of molecular cargo expands the possibilities of clinical therapy. This article explores the possibility of creating nanoparticles based on fucoidan modified with magnetite for biomedical purposes. The possibility of immobilizing a modelling fibrinolytic enzyme with a cross-linking agent was studied. The maximum loading of the enzyme is 2.06±0.09% of the mass. The particle size with immobilized alteplase according to scanning electron microscopy was 94.4±24.3 nm, hydrodynamic diameter - 370 nm, zeta potential – -1.66±0.06 mV. The saturation magnetization of the sample is 6 emu/g. To understand the mechanisms of molecular load release, five kinetic models were applied to the results obtained: zero order, Weibull, Hill equation, Higuchi, Korsmeyer-Peppas. The use of mathematical modeling showed that the best model for describing this process is the Korsmeyer-Peppas kinetic equation (r2 = 0.97), and the release process is controlled by the Fick diffusion. The resulting biocomposite material is a promising candidate as a nanocarrier for an enzymatic agent.