OPTIMIZATION OF THE SYNTHESIS METHOD FOR VITAMIN A NANOEMULSIONS

Abstract

The article presents the results of optimizing the procedure for the synthesis of vitamin A nanoemulsions. For the synthesis of nanoemulsions, vitamin A and Tween 80 were mixed, distilled water was added to the resulting mixture and mixed with a dispersant. To optimize the method for synthesizing vitamin A nanoemulsions, a multifactorial experiment was carried out, which included 4 input parameters. As input parameters, we considered the stirring speed, stirring time, the content of the dispersed phase, the content of fat-soluble vitamin A in the dispersed phase. The average hydrodynamic radius of vitamin A micelles was used as an output parameter. As a result of the studies, ternary surfaces of the dependence of the average hydrodynamic radius of vitamin A micelles on the parameters of nanoemulsion synthesis were obtained. It was found that all samples have a monomodal size distribution. The analysis of the data obtained made it possible to establish that the average hydrodynamic radius of vitamin A micelles is significantly affected by all the studied parameters. The optimal parameters for the synthesis of vitamin A nanoemulsions have been established: stirring speed (ν) - from 17000 to 22500 rpm, mixing time (τ) - from 180 to 270 s, content of the dispersed phase (ω (dispersed phase) - from 1 to 4%, the content of fat-soluble vitamin A in the dispersed phase (ω (vitamin A)) is from 50 to 70%. The nanoemulsion sample obtained with these parameters has an average hydrodynamic micelle radius of 62 ± 13 nm.