Enhancement of stability and activity of zinc carbonate nanoparticles using chitosan, hydroxyethyl cellulose, methyl cellulose and hyaluronic acid for multifaceted applications in medicine

Abstract

Currently, biopolymer-based Zn-containing nanoforms are of great interest for medical applications. However, there is lack information on optimal synthesis parameters, reagents and stabilizing agent for production of zinc carbonate nanoparticles (ZnC-NPs). In this work, synthesis of ZnC-NPs was carried out by chemical precipitation with the use of chitosan, hydroxyethyl cellulose, methyl cellulose and hyaluronic acid as stabilizing agents. The optimal precursor (Zn(CH3COO)2) and the optimal precipitator ((NH₄)₂CO₃) were detected. ZnC-NPs had one phase (Zn5(OH)6(CO3)2) with diameter from 35 to 120 nm. Thus, the optimal synthesis parameters were set as stoichiometric ratio of precursor and precipitator and the maximum concentration of biopolymer. It was found that polymers are sorbed on different crystallographic planes of crystallites, which affects the morphology of Zn5(OH)6(CO3). Quantum chemical modelling revealed that all models of interaction are energetically advantageous (∆E > 9788.910 kcal/mol) and preferably occurs through OH group, which was confirmed by FTIR spectroscopy of synthesized samples. Notably, CAM assay and histological evaluation showed that ZnC-NPs stabilized with chitosan (as represent of considered biopolymers) have no toxic effect and are compatible with CAM biological environment, which open a great potential for further studies of ZnC-NPs stabilized with biopolymers for multifaceted applications in medicine.