Please use this identifier to cite or link to this item: https://dspace.ncfu.ru/handle/20.500.12258/26861
Title: Composite of bacterial cellulose and gelatin: A versatile biocompatible scaffold for tissue engineering
Authors: Rzhepakovsky, I. V.
Ржепаковский, И. В.
Piskov, S. I.
Писков, С. И.
Avanesyan, S. S.
Аванесян, С. С.
Sizonenko, M. N.
Сизоненко, М. Н.
Timchenko, L. D.
Тимченко, Л. Д.
Anfinogenova, O. I.
Анфиногенова, О. И.
Nagdalian, A. A.
Нагдалян, А. А.
Blinov, A. V.
Блинов, А. В.
Denisova, E. V.
Денисова, Е. В.
Kochergin, S. G.
Кочергин, С. Г.
Kubanov, S. I.
Кубанов, С. И.
Shakhbanov, M. S.
Шахбанов, М. Ш.
Keywords: Microcomputed tomography;Tissue engineering;Bacterial cellulose;Biocompatibility;Scaffold
Issue Date: 2024
Citation: Rzhepakovsky, I., Piskov, S., Avanesyan, S., Sizonenko, M., Timchenko, L., Anfinogenova, O., Nagdalian, A., Blinov, A., Denisova, E., Kochergin, S., Kubanov, S., Shakhbanov, M., Shariati, M.A., Mubarak, M.S. Composite of bacterial cellulose and gelatin: A versatile biocompatible scaffold for tissue engineering // International Journal of Biological Macromolecules. - 2024. - 256. - статья № 128369. - DOI: 10.1016/j.ijbiomac.2023.128369
Series/Report no.: International Journal of Biological Macromolecules
Abstract: Synthesis of 0.4 ± 0.03 g/L per day of pure and porous bacterial cellulose (BC) scaffolds (scaffBC) and BC scaffolds modified with gelatin (scaffBC/Gel) was carried out using the Medusomyces gisevii Sa-28 bacterial strain. FT-IR spectroscopy and X-ray diffraction analysis showed that the scaffolds largely consist of crystalline cellulose I (Iα, Iß). Heating of BC with gelatin to 60 °C with subsequent lyophilization led to its modification by adsorption and binding of low-molecular fractions of gelatin and the formation of small pores between the fibers, which increased the biocompatibility and solubility of BC. The solubility of scaffBC and scaffBC/Gel was 20.8 % and 44.4 %, respectively, which enhances degradation in vivo. Light microscopy, scanning electron microscopy, and microcomputed tomography showed a uniform distribution of pores with a diameter of 100–500 μm. The chicken chorioallantoic membrane (CAM) model and subcutaneous implantation in rats confirmed low immunogenicity and intense formation of collagen fibers in both scaffolds and active germination of new blood vessels in scaffBC and scaffBC/Gel. The proliferative cellular activity of fibroblasts confirmed the safety of scaffolds. Taken together, the results obtained show that scaffBC/Gel can be used for the engineering of hard and soft tissues, which opens opportunities for further research.
URI: http://hdl.handle.net/20.500.12258/26861
Appears in Collections:Статьи, проиндексированные в SCOPUS, WOS

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