DEVELOPMENT OF A BIOLOGICALLY ACTIVE NANOSYSTEM BASED ON RIBOFLAVIN, A MICROELEMENT OF COPPER AND L-LYSINE AMINO ACID

Abstract

А biologically active nanosystem based on riboflavin, copper microelement and the amino acid lysine copper lysinatoriboflavinate has been developed. The obtained sample of the biologically active additive was studied by a complex of modern methods of analysis, namely: spectrophotometry, scanning electron microscopy, spectrophotoluminescence spectroscopy. In addition, computer quantum chemical modeling was carried out. It has been established that the most energetically favorable model of a biologically active nanosystem based on riboflavin, a trace element of copper and lysine is a model in which riboflavin is bound to the copper ion through the enol oxygen at C2 and the neighboring nitrogen heteroatom N3, and lysine is bound to the copper ion through the carboxyl and amino groups in α position. As a result of the analysis of micrographs of a biologically active nanosystem based on riboflavin, copper microelement and lysine amino acid, it was found that the particles have an acicular structure, the width of individual crystals is from 100 to 300 nm, and the length reaches several microns. The energy-dispersive X-ray spectra of a biologically active nanosystem based on riboflavin, the microelement copper, and the amino acid lysine coincide with the theoretical concepts of the structure of the elements under consideration. The analysis did not show the presence of impurity compounds, which may allow us to judge the high purity of the developed compound.